LOCUS       pLV-[hsa-miR-599]        9149 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7070..7930
                     /label=Amp(R)
     promoter        8131..8671
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5675..5909
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6484..6939)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5981..6111
                     /label=SV40 pA
     misc_feature    6112..6325
                     /label=SV40 ori
     misc_feature    4996..5592
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3614
                     /label=intron
     CDS             3661..4986
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3404
                     /label=hsa-mir-599 precursor
BASE COUNT     2186 a      2363 c      2465 g      2135 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg ttcttaaaat 
     3121 cacctggaaa cactgaggtt gtgtctcact gaacataagc ttcgtgactt ctattcagaa 
     3181 ctttaatgac tgggaatacg tttaatgcca aagacatgct gtccacagtg tgtttgataa 
     3241 gctgacatgg gacagggatt cttttcactg ttgtgtcagt ttatcaaacc catacttgga 
     3301 tgacagtcaa ttcagttaaa ggataatggg gtcccaggat acaaaacatt atttccttta 
     3361 cagaagctat gcactctatg ttgtacaaat gccacacaca tatggctagc tcgagctttt 
     3421 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 
     3481 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 
     3541 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 
     3601 ttcttccatt tcaggtgtcg tgaaaactac ccctctagag tcgagctacc ggtcgccacc 
     3661 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 
     3721 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 
     3781 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 
     3841 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 
     3901 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 
     3961 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 
     4021 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 
     4081 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 
     4141 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 
     4201 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 
     4261 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 
     4321 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagga cccaccggtc 
     4381 gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 
     4441 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 
     4501 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 
     4561 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 
     4621 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 
     4681 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 
     4741 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 
     4801 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 
     4861 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 
     4921 accgccgacg tcgagtgccc gaaggaccgc gcgacctggt gcatgacccg caagcccggt 
     4981 gcctgagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg 
     5041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     5101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     5161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     5221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     5281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     5341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 
     5401 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     5461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     5521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     5581 cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt 
     5641 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 
     5701 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 
     5761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     5821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     5881 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 
     5941 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 
     6001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     6061 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 
     6121 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     6181 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     6241 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct 
     6301 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     6361 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     6421 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     6481 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     6541 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     6601 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     6661 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     6721 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     6781 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     6841 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     6901 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 
     6961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     7021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     7081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     7141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     7201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     7261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     7321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     7381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     7441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     7501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     7561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     7621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     7681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     7741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 
     7801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     7861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     7921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     7981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     8041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     8101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     8161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     8221 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     8281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     8341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     8401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     8461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     8521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     8581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     8641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     8701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     8761 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
     8821 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 
     8881 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
     8941 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
     9001 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
     9061 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 
     9121 tcactaaagg gaacaaaagc tggagctgc 
//
